Journal: Nanomaterials

Article Title: Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo

doi: 10.3390/nano13030372

Figure Lengend Snippet: Inhibition of melanoma cells proliferation in vivo by HPL, 3HFWC, and HPL+3HFWC treatments, as demonstrated by ( A ) PCNA immunoexpression (green) in melanoma tissue, red signal—propidium iodide nuclear staining (scale bar and orig. magnification—25 μm, × 63); ( B ) PCNA nuclear immunopositivity levels (0/+ no/weak immunopositivity; ++/+++ medium/strong immunopositivity of nuclei); and ( C ) mitotic index in melanoma tissue. Mean values ± SEM; statistical significance in comparison to melanoma tissue of control animals (* p value) and between the treated groups.

Article Snippet: After deparaffinization and rehydration, epitope retrieval was performed by microwave treatment in citrate buffer for 5 min. After cooling and rinsing with distilled water and PBS, nonspecific binding was blocked by using 5% bovine serum albumin (BSA) at room temperature, for 30 min. A rabbit polyclonal antibody against PCNA was used (eBioscience, #60053, 1/100, overnight, at 4 °C), followed by thorough washing in PBS with Tween 20.

Techniques: Inhibition, In Vivo, Staining, Comparison, Control